Anti-Phospho-MeCP2 (Ser80) Antibody

Product Name

Anti-Phospho-MeCP2 (Ser80) Antibody

View all MECP2 Antibodies

SKU/Catalog Number

P00047-1

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-MeCP2 (Ser80) Antibody (Catalog # P00047-1). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-MeCP2 (Ser80) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00047-1)

Host

Rabbit

Contents

Serum

Clonality

Polyclonal

Clone Number

JE44-80

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 of human MeCP2, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00047-1 is reactive to MECP2 in Human, Mouse, Rat

Applications

P00047-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

52.441kDa

Background of MECP2

MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:100-1:250

Validation Images & Assay Conditions

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Western blot of neonatal rat brain showing specific immunolabeling of the ~75 kDa MeCP2 protein phosphorylated at Ser⁸⁰.

Gene/Protein Information For MECP2 (Source: Uniprot.org, NCBI)

Alternative Names

AUTSX3; DKFZp686A24160; MeCp-2 protein; mental retardation, X-linked 79; methyl CpG binding protein 2 (Rett syndrome); X-linked 16 MECP2 AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT methyl-CpG binding protein 2 methyl-CpG-binding protein 2|meCp-2 protein

For more info on MECP2, check out the MECP2 Infographic

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In this infographic, you will see the following information for MECP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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